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Product Details:
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Apperance: | White Powder | Shelf Life: | 2 Years |
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Product Information
Basic information:
Product Name: Recombinant Bovine Enterokinase ( rb-EK)
Catalog Number: YSRBEK
Packing Details: 200 U, 4 KU, 40 KU
Formulation: Lyophilized from 50 mM Tris-HCl, pH8.0
Mol. Wt.: 26.2 kDa, 30 kDa by SDS-PAGE analysis
Theoretical pI: 5.20
Activity: ≥40 KU/mg
Purity: ≥95% by SDS-PAGE analysis
Endotoxin: ≤5 EU/mg
Resources: Escherichia coli (E. coli)
Storage Condition: -20 oC
Storage Duration: 3 years
Unit Definition: 1 unit is defined as the amount of rb-EK that will digest 50 μg of 1mg/ml fusion protein to 95% completion in 16 h at 25oC in 50 mM Tris-HCl, pH 8.0.
Description:
Enterokinase is a serine protease that can specifically cleave the sequence Asp-Asp-Asp-Asp-Lys after the lysine residue . It applies for digesting fusion proteins to release the fusion partner or tag from the target protein.
Native enterokinase is composed of a 115 kDa heavy chain as a membrane anchor and a 35 kDa light chain as the catalytic subunit. Recombinant bovine enterokinase is a specially prepared recombinant enzyme, consisting of the catalytic subunit of the holoenzyme. This subunit is expressed and purified from E.coli, yielding an enzyme with higher specific activity. This results in more
efficient cleavage using less enzyme.
Note:
1. Rb-EK is known to be inhibited by >2 M Urea, >250 mM NaCl, >20 mM β-ME, >0.1% SDS, or >50 mM imidazole. Dialyze your fusion protein to remove the inhibitors prior to digestion with rb-EK.
2. Avoid using phosphate buffer.
Key words:
Recombinant Bovine Enterokinase
rb-EK
Enterokinase
Bovine Enterokinase
Recombinant Enterokinase
Company Information
Chengdu Youngshe Chemical Co.,Ltd, is a dynamic and progressive peptides manufacturer in China. We are dedicating to be a professional, efficient,and reliable partner for global customers on peptides.Hi-new tech Zone campus in the suburb of Chengdu city provides Youngshe with a good infrastructure for both industrial-scale production of peptides and custom peptide synthesis.You can select more than 200 kinds of raw cosmetic peptide ingredients here.Youngshe provide a one-stop service for raw cosmetic peptide ingredients.You will save lots of time ,energy, money and have a very pleasant experience with us.
Our Advantages
1.Competitive factory price.
2. Professionalism of production: With more than 10 years of experience in peptide production, to ensure the stability and reliability of each batch of peptide quality.
3. Professionalism of product efficacy: We are familiar with thousands of peptides that are now available worldwide, thorough research.
4. Professionalism of service:customer satisfaction is our greatest work.
5. Reliability: We always believe that only good reputation and product quality can make a good company.
6. Flexibility:We offer a variety of peptides, liquid, freeze-dried powder etc.
7. Variety of products: We offer more than 200 cosmetic peptides, including Dipeptide, Tripeptide, Tetrapeptide, Pentapeptide, Hexapeptide, Heptapeptide, Octapeptide, Nonapeptide, Decapeptide, Copper peptide, Oligopeptide and so on.
Packing&Shipping
F&Q
Q1: How do I make an order?
A: Simply send an inquiry to contact with us and we will be happy to assist you with your order.
Q2: How to start orders or make payments?
A: Proforma invoice will be sent first after confirmation of order, enclosed our bank information.We accept T/T (Telegraphic
Transfer),Western Union or Paypal.
Q3: How about delivery lead time?
A:Delivery lead time: About 3-5 days after payment confirmed. (Chinese holiday not included)
Q4:Is there a discount?
A:Different quantity has different discount.
Q5: How do you treat quality complaint?
A:First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us,
we will send you free goods for replacement or refund your loss.
Contact info.
Aileen Chen
Email: aileen@youngshechem.com
Tel/whatsapp/Skype/WeChat: +8619150309904
Contact Person: Ms. Aileen Chen
Tel: +8619150309904
Fax: 86-28-62328193